Dynamics of the Phanerochaete carnosa transcriptome during growth on aspen and spruce.
Identifieur interne : 000F46 ( Main/Exploration ); précédent : 000F45; suivant : 000F47Dynamics of the Phanerochaete carnosa transcriptome during growth on aspen and spruce.
Auteurs : E. Jurak [Finlande, Pays-Bas] ; H. Suzuki [Canada] ; G. Van Erven [Pays-Bas] ; J A Gandier [Canada] ; P. Wong [Canada] ; K. Chan [Canada] ; C Y Ho [Canada] ; Y. Gong [Canada] ; E. Tillier [Canada] ; M-N Rosso [France] ; M A Kabel [Pays-Bas] ; S. Miyauchi [France] ; E R Master [Finlande, Canada]Source :
- BMC genomics [ 1471-2164 ] ; 2018.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Phanerochaete, Protéines fongiques.
- microbiologie : Bois, Picea, Populus.
- physiologie : Phanerochaete.
- Analyse de profil d'expression de gènes, Régulation de l'expression des gènes fongiques, Transcriptome.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Fungal Proteins.
- genetics : Phanerochaete.
- microbiology : Picea, Populus, Wood.
- physiology : Phanerochaete.
- Gene Expression Profiling, Gene Expression Regulation, Fungal, Transcriptome.
Abstract
BACKGROUND
The basidiomycete Phanerochaete carnosa is a white-rot species that has been mainly isolated from coniferous softwood. Given the particular recalcitrance of softwoods to bioconversion, we conducted a comparative transcriptomic analysis of P. carnosa following growth on wood powder from one softwood (spruce; Picea glauca) and one hardwood (aspen; Populus tremuloides). P. carnosa was grown on each substrate for over one month, and mycelia were harvested at five time points for total RNA sequencing. Residual wood powder was also analyzed for total sugar and lignin composition.
RESULTS
Following a slightly longer lag phase of growth on spruce, radial expansion of the P. carnosa colony was similar on spruce and aspen. Consistent with this observation, the pattern of gene expression by P. carnosa on each substrate converged following the initial adaptation. On both substrates, highest transcript abundances were attributed to genes predicted to encode manganese peroxidases (MnP), along with auxiliary activities from carbohydrate-active enzyme (CAZy) families AA3 and AA5. In addition, a lytic polysaccharide monooxygenase from family AA9 was steadily expressed throughout growth on both substrates. P450 sequences from clans CPY52 and CYP64 accounted for 50% or more of the most highly expressed P450s, which were also the P450 clans that were expanded in the P. carnosa genome relative to other white-rot fungi.
CONCLUSIONS
The inclusion of five growth points and two wood substrates was important to revealing differences in the expression profiles of specific sequences within large glycoside hydrolase families (e.g., GH5 and GH16), and permitted co-expression analyses that identified new targets for study, including non-catalytic proteins and proteins with unknown function.
DOI: 10.1186/s12864-018-5210-z
PubMed: 30424733
PubMed Central: PMC6234650
Affiliations:
- Canada, Finlande, France, Pays-Bas
- Groningue (province), Gueldre (province), Ontario, Provence-Alpes-Côte d'Azur, Uusimaa
- Espoo, Groningue, Marseille, Toronto, Wageningue
- Université Aalto, Université d'Aix-Marseille, Université de Toronto, Université de Wageningue
Links toward previous steps (curation, corpus...)
Le document en format XML
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Jurak</name><affiliation wicri:level="4"><nlm:affiliation>Department of Bioproducts and Biosystems, Aalto University, Espoo, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Bioproducts and Biosystems, Aalto University, Espoo</wicri:regionArea><orgName type="university">Université Aalto</orgName><placeName><settlement type="city">Espoo</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Department of Aquatic Biotechnology and Bioproduct Engineering, Groningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Aquatic Biotechnology and Bioproduct Engineering, Groningen</wicri:regionArea><placeName><settlement type="city">Groningue</settlement><region nuts="2" type="province">Groningue (province)</region></placeName></affiliation></author><author><name sortKey="Suzuki, H" sort="Suzuki, H" uniqKey="Suzuki H" first="H" last="Suzuki">H. 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Wong</name><affiliation wicri:level="4"><nlm:affiliation>Department of Medical Biophysics, University of Toronto, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Medical Biophysics, University of Toronto, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Chan, K" sort="Chan, K" uniqKey="Chan K" first="K" last="Chan">K. Chan</name><affiliation wicri:level="3"><nlm:affiliation>Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Ho, C Y" sort="Ho, C Y" uniqKey="Ho C" first="C Y" last="Ho">C Y Ho</name><affiliation wicri:level="3"><nlm:affiliation>Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Gong, Y" sort="Gong, Y" uniqKey="Gong Y" first="Y" last="Gong">Y. Gong</name><affiliation wicri:level="4"><nlm:affiliation>Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Tillier, E" sort="Tillier, E" uniqKey="Tillier E" first="E" last="Tillier">E. Tillier</name><affiliation wicri:level="4"><nlm:affiliation>Department of Medical Biophysics, University of Toronto, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Medical Biophysics, University of Toronto, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Rosso, M N" sort="Rosso, M N" uniqKey="Rosso M" first="M-N" last="Rosso">M-N Rosso</name><affiliation wicri:level="4"><nlm:affiliation>Aix-Marseille Université, INRA, UMR1163, Biodiversité et Biotechnologie Fongiques, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix-Marseille Université, INRA, UMR1163, Biodiversité et Biotechnologie Fongiques, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Kabel, M A" sort="Kabel, M A" uniqKey="Kabel M" first="M A" last="Kabel">M A Kabel</name><affiliation wicri:level="4"><nlm:affiliation>Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708, WG, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708, WG, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="Miyauchi, S" sort="Miyauchi, S" uniqKey="Miyauchi S" first="S" last="Miyauchi">S. Miyauchi</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Excellence ARBRE, INRA, Nancy, Lorraine, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Excellence ARBRE, INRA, Nancy, Lorraine</wicri:regionArea><wicri:noRegion>Lorraine</wicri:noRegion><wicri:noRegion>Lorraine</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:affiliation>Aix-Marseille Université, INRA, UMR1163, Biodiversité et Biotechnologie Fongiques, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix-Marseille Université, INRA, UMR1163, Biodiversité et Biotechnologie Fongiques, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Master, E R" sort="Master, E R" uniqKey="Master E" first="E R" last="Master">E R Master</name><affiliation wicri:level="4"><nlm:affiliation>Department of Bioproducts and Biosystems, Aalto University, Espoo, Finland. emma.master@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Bioproducts and Biosystems, Aalto University, Espoo</wicri:regionArea><orgName type="university">Université Aalto</orgName><placeName><settlement type="city">Espoo</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada. emma.master@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:30424733</idno><idno type="pmid">30424733</idno><idno type="doi">10.1186/s12864-018-5210-z</idno><idno type="pmc">PMC6234650</idno><idno type="wicri:Area/Main/Corpus">000B80</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000B80</idno><idno type="wicri:Area/Main/Curation">000B80</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000B80</idno><idno type="wicri:Area/Main/Exploration">000B80</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Dynamics of the Phanerochaete carnosa transcriptome during growth on aspen and spruce.</title><author><name sortKey="Jurak, E" sort="Jurak, E" uniqKey="Jurak E" first="E" last="Jurak">E. Jurak</name><affiliation wicri:level="4"><nlm:affiliation>Department of Bioproducts and Biosystems, Aalto University, Espoo, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Bioproducts and Biosystems, Aalto University, Espoo</wicri:regionArea><orgName type="university">Université Aalto</orgName><placeName><settlement type="city">Espoo</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Department of Aquatic Biotechnology and Bioproduct Engineering, Groningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Aquatic Biotechnology and Bioproduct Engineering, Groningen</wicri:regionArea><placeName><settlement type="city">Groningue</settlement><region nuts="2" type="province">Groningue (province)</region></placeName></affiliation></author><author><name sortKey="Suzuki, H" sort="Suzuki, H" uniqKey="Suzuki H" first="H" last="Suzuki">H. Suzuki</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Van Erven, G" sort="Van Erven, G" uniqKey="Van Erven G" first="G" last="Van Erven">G. Van Erven</name><affiliation wicri:level="4"><nlm:affiliation>Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708, WG, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708, WG, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="Gandier, J A" sort="Gandier, J A" uniqKey="Gandier J" first="J A" last="Gandier">J A Gandier</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Wong, P" sort="Wong, P" uniqKey="Wong P" first="P" last="Wong">P. Wong</name><affiliation wicri:level="4"><nlm:affiliation>Department of Medical Biophysics, University of Toronto, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Medical Biophysics, University of Toronto, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Chan, K" sort="Chan, K" uniqKey="Chan K" first="K" last="Chan">K. Chan</name><affiliation wicri:level="3"><nlm:affiliation>Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Ho, C Y" sort="Ho, C Y" uniqKey="Ho C" first="C Y" last="Ho">C Y Ho</name><affiliation wicri:level="3"><nlm:affiliation>Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Gong, Y" sort="Gong, Y" uniqKey="Gong Y" first="Y" last="Gong">Y. Gong</name><affiliation wicri:level="4"><nlm:affiliation>Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Tillier, E" sort="Tillier, E" uniqKey="Tillier E" first="E" last="Tillier">E. Tillier</name><affiliation wicri:level="4"><nlm:affiliation>Department of Medical Biophysics, University of Toronto, Toronto, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Medical Biophysics, University of Toronto, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Rosso, M N" sort="Rosso, M N" uniqKey="Rosso M" first="M-N" last="Rosso">M-N Rosso</name><affiliation wicri:level="4"><nlm:affiliation>Aix-Marseille Université, INRA, UMR1163, Biodiversité et Biotechnologie Fongiques, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix-Marseille Université, INRA, UMR1163, Biodiversité et Biotechnologie Fongiques, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Kabel, M A" sort="Kabel, M A" uniqKey="Kabel M" first="M A" last="Kabel">M A Kabel</name><affiliation wicri:level="4"><nlm:affiliation>Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708, WG, Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708, WG, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="Miyauchi, S" sort="Miyauchi, S" uniqKey="Miyauchi S" first="S" last="Miyauchi">S. Miyauchi</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Excellence ARBRE, INRA, Nancy, Lorraine, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Excellence ARBRE, INRA, Nancy, Lorraine</wicri:regionArea><wicri:noRegion>Lorraine</wicri:noRegion><wicri:noRegion>Lorraine</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:affiliation>Aix-Marseille Université, INRA, UMR1163, Biodiversité et Biotechnologie Fongiques, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Aix-Marseille Université, INRA, UMR1163, Biodiversité et Biotechnologie Fongiques, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Master, E R" sort="Master, E R" uniqKey="Master E" first="E R" last="Master">E R Master</name><affiliation wicri:level="4"><nlm:affiliation>Department of Bioproducts and Biosystems, Aalto University, Espoo, Finland. emma.master@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Bioproducts and Biosystems, Aalto University, Espoo</wicri:regionArea><orgName type="university">Université Aalto</orgName><placeName><settlement type="city">Espoo</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada. emma.master@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto</wicri:regionArea><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author></analytic><series><title level="j">BMC genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fungal Proteins (genetics)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Phanerochaete (genetics)</term><term>Phanerochaete (physiology)</term><term>Picea (microbiology)</term><term>Populus (microbiology)</term><term>Transcriptome (MeSH)</term><term>Wood (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Bois (microbiologie)</term><term>Phanerochaete (génétique)</term><term>Phanerochaete (physiologie)</term><term>Picea (microbiologie)</term><term>Populus (microbiologie)</term><term>Protéines fongiques (génétique)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phanerochaete</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Bois</term><term>Picea</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Picea</term><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Fungal</term><term>Transcriptome</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Régulation de l'expression des gènes fongiques</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>The basidiomycete Phanerochaete carnosa is a white-rot species that has been mainly isolated from coniferous softwood. Given the particular recalcitrance of softwoods to bioconversion, we conducted a comparative transcriptomic analysis of P. carnosa following growth on wood powder from one softwood (spruce; Picea glauca) and one hardwood (aspen; Populus tremuloides). P. carnosa was grown on each substrate for over one month, and mycelia were harvested at five time points for total RNA sequencing. Residual wood powder was also analyzed for total sugar and lignin composition.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Following a slightly longer lag phase of growth on spruce, radial expansion of the P. carnosa colony was similar on spruce and aspen. Consistent with this observation, the pattern of gene expression by P. carnosa on each substrate converged following the initial adaptation. On both substrates, highest transcript abundances were attributed to genes predicted to encode manganese peroxidases (MnP), along with auxiliary activities from carbohydrate-active enzyme (CAZy) families AA3 and AA5. In addition, a lytic polysaccharide monooxygenase from family AA9 was steadily expressed throughout growth on both substrates. P450 sequences from clans CPY52 and CYP64 accounted for 50% or more of the most highly expressed P450s, which were also the P450 clans that were expanded in the P. carnosa genome relative to other white-rot fungi.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>The inclusion of five growth points and two wood substrates was important to revealing differences in the expression profiles of specific sequences within large glycoside hydrolase families (e.g., GH5 and GH16), and permitted co-expression analyses that identified new targets for study, including non-catalytic proteins and proteins with unknown function.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30424733</PMID><DateCompleted><Year>2019</Year><Month>02</Month><Day>06</Day></DateCompleted><DateRevised><Year>2019</Year><Month>02</Month><Day>15</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2164</ISSN><JournalIssue CitedMedium="Internet"><Volume>19</Volume><Issue>1</Issue><PubDate><Year>2018</Year><Month>Nov</Month><Day>13</Day></PubDate></JournalIssue><Title>BMC genomics</Title><ISOAbbreviation>BMC Genomics</ISOAbbreviation></Journal><ArticleTitle>Dynamics of the Phanerochaete carnosa transcriptome during growth on aspen and spruce.</ArticleTitle><Pagination><MedlinePgn>815</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12864-018-5210-z</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The basidiomycete Phanerochaete carnosa is a white-rot species that has been mainly isolated from coniferous softwood. Given the particular recalcitrance of softwoods to bioconversion, we conducted a comparative transcriptomic analysis of P. carnosa following growth on wood powder from one softwood (spruce; Picea glauca) and one hardwood (aspen; Populus tremuloides). P. carnosa was grown on each substrate for over one month, and mycelia were harvested at five time points for total RNA sequencing. Residual wood powder was also analyzed for total sugar and lignin composition.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Following a slightly longer lag phase of growth on spruce, radial expansion of the P. carnosa colony was similar on spruce and aspen. Consistent with this observation, the pattern of gene expression by P. carnosa on each substrate converged following the initial adaptation. On both substrates, highest transcript abundances were attributed to genes predicted to encode manganese peroxidases (MnP), along with auxiliary activities from carbohydrate-active enzyme (CAZy) families AA3 and AA5. In addition, a lytic polysaccharide monooxygenase from family AA9 was steadily expressed throughout growth on both substrates. P450 sequences from clans CPY52 and CYP64 accounted for 50% or more of the most highly expressed P450s, which were also the P450 clans that were expanded in the P. carnosa genome relative to other white-rot fungi.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The inclusion of five growth points and two wood substrates was important to revealing differences in the expression profiles of specific sequences within large glycoside hydrolase families (e.g., GH5 and GH16), and permitted co-expression analyses that identified new targets for study, including non-catalytic proteins and proteins with unknown function.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jurak</LastName><ForeName>E</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Bioproducts and Biosystems, Aalto University, Espoo, Finland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Aquatic Biotechnology and Bioproduct Engineering, Groningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Suzuki</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van Erven</LastName><ForeName>G</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708, WG, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gandier</LastName><ForeName>J A</ForeName><Initials>JA</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wong</LastName><ForeName>P</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Medical Biophysics, University of Toronto, Toronto, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chan</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ho</LastName><ForeName>C Y</ForeName><Initials>CY</Initials><AffiliationInfo><Affiliation>Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gong</LastName><ForeName>Y</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tillier</LastName><ForeName>E</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Medical Biophysics, University of Toronto, Toronto, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rosso</LastName><ForeName>M-N</ForeName><Initials>MN</Initials><AffiliationInfo><Affiliation>Aix-Marseille Université, INRA, UMR1163, Biodiversité et Biotechnologie Fongiques, Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kabel</LastName><ForeName>M A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Wageningen University, Laboratory of Food Chemistry, Bornse Weilanden 9, 6708, WG, Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miyauchi</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratory of Excellence ARBRE, INRA, Nancy, Lorraine, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Aix-Marseille Université, INRA, UMR1163, Biodiversité et Biotechnologie Fongiques, Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Master</LastName><ForeName>E R</ForeName><Initials>ER</Initials><AffiliationInfo><Affiliation>Department of Bioproducts and Biosystems, Aalto University, Espoo, Finland. emma.master@utoronto.ca.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada. emma.master@utoronto.ca.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>ORF-RE-05-005</GrantID><Agency>Government of Ontario</Agency><Country></Country></Grant><Grant><GrantID>648925</GrantID><Agency>European Research Council</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>11</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Genomics</MedlineTA><NlmUniqueID>100965258</NlmUniqueID><ISSNLinking>1471-2164</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="Y">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028222" MajorTopicYN="N">Picea</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="Y">Transcriptome</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Carbohydrate active enzymes</Keyword><Keyword MajorTopicYN="N">Hydrophobins</Keyword><Keyword MajorTopicYN="N">Lignocellulose conversions</Keyword><Keyword MajorTopicYN="N">Loosenins</Keyword><Keyword MajorTopicYN="N">Phanerochaete carnosa</Keyword><Keyword MajorTopicYN="N">Transcriptomics</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>06</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>10</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>11</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>11</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>2</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30424733</ArticleId><ArticleId IdType="doi">10.1186/s12864-018-5210-z</ArticleId><ArticleId IdType="pii">10.1186/s12864-018-5210-z</ArticleId><ArticleId IdType="pmc">PMC6234650</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Fungal Genet Biol. 2014 Nov;72:91-98</Citation><ArticleIdList><ArticleId IdType="pubmed">24394946</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biotechnol. 2007 Feb 20;128(3):500-11</Citation><ArticleIdList><ArticleId IdType="pubmed">17218034</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2016 Sep 05;9(1):192</Citation><ArticleIdList><ArticleId IdType="pubmed">27602055</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11295-9</Citation><ArticleIdList><ArticleId IdType="pubmed">1837148</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2014 Apr;80(7):2062-70</Citation><ArticleIdList><ArticleId IdType="pubmed">24441164</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2014 Nov;72:115-123</Citation><ArticleIdList><ArticleId IdType="pubmed">24880035</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2010 Jan 1;26(1):139-40</Citation><ArticleIdList><ArticleId IdType="pubmed">19910308</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2011 May;77(10):3211-8</Citation><ArticleIdList><ArticleId IdType="pubmed">21441342</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2018 Sep;95(6):1114-1128</Citation><ArticleIdList><ArticleId IdType="pubmed">29932263</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Microbiol. 2017 Mar;19(3):1237-1250</Citation><ArticleIdList><ArticleId IdType="pubmed">28028889</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2004 Jun;22(6):695-700</Citation><ArticleIdList><ArticleId IdType="pubmed">15122302</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2014 Nov;72:82-90</Citation><ArticleIdList><ArticleId IdType="pubmed">24853079</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2016 Jun 13;9:124</Citation><ArticleIdList><ArticleId IdType="pubmed">27303445</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Bioinformatics. 2003 Sep 11;4:41</Citation><ArticleIdList><ArticleId IdType="pubmed">12969510</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2017 Jun 20;8(3):</Citation><ArticleIdList><ArticleId IdType="pubmed">28634239</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Appl Biochem. 2013 Jan-Feb;60(1):71-81</Citation><ArticleIdList><ArticleId IdType="pubmed">23586994</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol Biotechnol. 2017 Mar;101(6):2343-2356</Citation><ArticleIdList><ArticleId IdType="pubmed">27917435</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biotechnol. 1999 Sep 24;75(1):57-70</Citation><ArticleIdList><ArticleId IdType="pubmed">10704993</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Microbiol. 2016 Sep;18(9):1285-93</Citation><ArticleIdList><ArticleId IdType="pubmed">27306610</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Evol Biol. 2012 Sep 20;12:186</Citation><ArticleIdList><ArticleId IdType="pubmed">22992189</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2015 Oct 05;10(10):e0138909</Citation><ArticleIdList><ArticleId IdType="pubmed">26436656</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2012 Sep 02;13:444</Citation><ArticleIdList><ArticleId IdType="pubmed">22937793</ArticleId></ArticleIdList></Reference><Reference><Citation>Microb Cell Fact. 2011 Feb 11;10:8</Citation><ArticleIdList><ArticleId IdType="pubmed">21314954</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2017 Jan 4;45(D1):D190-D199</Citation><ArticleIdList><ArticleId IdType="pubmed">27899635</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2010 Jul;101(13):4992-5002</Citation><ArticleIdList><ArticleId IdType="pubmed">19969450</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2006 Jul;72(7):4871-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16820482</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2015 Dec 18;8:216</Citation><ArticleIdList><ArticleId IdType="pubmed">26692083</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2012 Jun 13;60(23):5922-35</Citation><ArticleIdList><ArticleId IdType="pubmed">22607527</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2014 Nov;72:124-130</Citation><ArticleIdList><ArticleId IdType="pubmed">24915038</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2013 Mar 21;6(1):41</Citation><ArticleIdList><ArticleId IdType="pubmed">23514094</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Chem. 2017 Oct 17;89(20):10907-10916</Citation><ArticleIdList><ArticleId IdType="pubmed">28926698</ArticleId></ArticleIdList></Reference><Reference><Citation>Microb Biotechnol. 2013 May;6(3):248-63</Citation><ArticleIdList><ArticleId IdType="pubmed">23279857</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2017 Apr 10;12(4):e0175528</Citation><ArticleIdList><ArticleId IdType="pubmed">28394946</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2000 Sep 21;407(6802):321-6</Citation><ArticleIdList><ArticleId IdType="pubmed">11014181</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2011 Jul;77(13):4499-507</Citation><ArticleIdList><ArticleId IdType="pubmed">21551287</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2018 Mar;112:47-54</Citation><ArticleIdList><ArticleId IdType="pubmed">28754284</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2018 Aug 1;84(16):</Citation><ArticleIdList><ArticleId IdType="pubmed">29884757</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2012 Mar;78(5):1596-600</Citation><ArticleIdList><ArticleId IdType="pubmed">22210217</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2015 Jan;43(Database issue):D1049-56</Citation><ArticleIdList><ArticleId IdType="pubmed">25428369</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 2018 Mar;112:40-46</Citation><ArticleIdList><ArticleId IdType="pubmed">28803908</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Nov 20;8(11):e80298</Citation><ArticleIdList><ArticleId IdType="pubmed">24278272</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Biochem Biotechnol. 2004 Apr;117(1):1-17</Citation><ArticleIdList><ArticleId IdType="pubmed">15126700</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1999 Jan 1;27(1):29-34</Citation><ArticleIdList><ArticleId IdType="pubmed">9847135</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Chem Biol. 2018 Mar;14(3):306-310</Citation><ArticleIdList><ArticleId IdType="pubmed">29377002</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2016 Aug 24;7:1335</Citation><ArticleIdList><ArticleId IdType="pubmed">27605927</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Rev. 2005 Nov;29(5):877-96</Citation><ArticleIdList><ArticleId IdType="pubmed">16219510</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2015 Nov;81(22):7802-12</Citation><ArticleIdList><ArticleId IdType="pubmed">26341198</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Microbiol. 2001;55:625-46</Citation><ArticleIdList><ArticleId IdType="pubmed">11544369</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2014;15(12):550</Citation><ArticleIdList><ArticleId IdType="pubmed">25516281</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>Finlande</li><li>France</li><li>Pays-Bas</li></country><region><li>Groningue (province)</li><li>Gueldre (province)</li><li>Ontario</li><li>Provence-Alpes-Côte d'Azur</li><li>Uusimaa</li></region><settlement><li>Espoo</li><li>Groningue</li><li>Marseille</li><li>Toronto</li><li>Wageningue</li></settlement><orgName><li>Université Aalto</li><li>Université d'Aix-Marseille</li><li>Université de Toronto</li><li>Université de Wageningue</li></orgName></list><tree><country name="Finlande"><region name="Uusimaa"><name sortKey="Jurak, E" 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